Troubleshooting Protein Expression

Summary

Today I thought I'd go over some background and practical advice for things you can do if you ask a cell to make a Some background & practical advice for things you can do if you ask a cell to make a There are lots of ways to end up with no E. coli bacteria are a common host for the It's good practice to take samples throughout the procedure so you can check afterwards how your Do you wish there were an easy way to optimize No Western blot band for your target—even though your controls look fine? Before concluding that the The basic principle behind recombinant Presented By: Alex Kirkpatrick, Ph.D. & Joanna Geddes Webinar: Optimizing Produced and created by Allysha O'donnell as part of the Advanced Chemistry Communications CHM 5500 course taught by Dr. Here's an overview of some of the common techniques used to express and purify In this video, I lay out the basics of how to transform bacteria and express a